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The ascomycete family Nectriaceae Hypocreales includes numerous important plant and human pathogens, as well as several species used extensively in industrial and commercial applications as biodegraders and biocontrol agents. Members of the family are unified by phenotypic characters such as uniloculate ascomata that are yellow, orange-red to purple, and with phialidic asexual morphs. The generic concepts in Nectriaceae are poorly defined, since DNA sequence data have not been available for many of these genera. To address this issue we performed a multi-gene phylogenetic analysis using partial sequences for the Better first dates - Saskatoon guy for older ladies large subunit LSU nrDNA, the internal transcribed spacer region and intervening 5.

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Syaranamual, Poka, Tlk. S1 The occurrence of Heteralepas japonica in the Moluccas red dots ; Fig. S2 The occurrence of Amphibalanus reticulatusDosima fascicularisEuraphia hembeliHexechamaesipho pilsbryiMegabalanus zebraNesochthamalus intertextusNewmanella spinosusPseudoctomeris sulcataTetraclitella divisa and Tetraclitella karandei in Girls for sex from Brookings South Dakota Moluccas the red dot indicates that the species occurs on the coastline of the island ; Fig.

S3 The occurrence of Chthamalus moro and Lepas anserifera in the Moluccas the red dots indicate that the species occurs on Hot woman wants nsa Carolina coastline of the islands ; Fig. S4 The occurrence of Amphibalanus amphitriteAmphibalanus variegatusCapitulum mitellaMegabalanus tintinnabulumTesseropora roseaTetraclita kuroshioensis and Tetraclita squamosa in the Moluccas the red dots indicate that the species occurs on the coastline of the islands ; Fig. S5 The occurrence of Microeuraphia sp. S6 The occurrence of Yamaguchiella coerulescens in the Moluccas the red dots indicate that the species occurs on the coastline of the islands ; Fig.

S7 The occurrence of Neonrosella vitiata in the Moluccas the red dots indicate that the species occurs on the coastline of the islands ; Fig. S8 The occurrence of Amphibalanus zhujiangensis in the Moluccas the red dots indicate that the species occurs on the coastline of the islands ; Fig. S9 The occurrence of Amphibalanus sp. Values next to nodes are support values; Fig. S12 ML phylogeny of 18S gene sequences. Tips coloured in red are the new samples sequenced for this manuscript.

Values next to nodes are support values; Table S1. Measurements for Microeuraphia sp. Measurements for Amphibalanus sp.

To contribute to the taxonomic knowledge of barnacles in this understudied area, the first checklist of barnacles from the Moluccas is presented, including additional information on morphology, distribution, and substrate as well as molecular data. The species of barnacles from the Moluccas have been determined using morphological analysis Woman looking real sex Blue Mounds DNA sequences. During 19 field trips conducted between January and September1, specimens of 24 species of intertidal and one species of deep-sea barnacles were collected from 51 localities from the islands.

Morphological and molecular analysis of the collected material detected members of three families of stalked barnacles and four families of acorn barnacles. In addition to sampling in the field, we also surveyed the Adult nursing relationship Gillette Wyoming on barnacles from the Moluccas. In total, our checklist comprises 97 species from the Moluccas including 23 new records, two of them yet to be described species.

suggest that the Moluccas have a much higher diversity of barnacles than ly known, for example, from the reports of Challenger and Siboga expeditions.

For further work, routine application of molecular systematics could aid the detection of cryptic species, while increased sampling of more islands and a taxonomic revision of several groups would likely lead to an even higher of species than currently known. Barnacles Crustacea Looking for sex in Braeside, Ontario, Cirripedia are an ancient, species-rich and abundant group of crustaceans with about 1, extant species Newman and Abbott They have a worldwide distribution in tropical and temperate marine environments and at different depths and are adjusted to various lifestyles, from parasites of decapod crustaceans to free-living groups.

Most cirripeds usually have two free-swimming planktonic larval stages consisting of distinctive nauplii and a unique non-feeding cyprid Darwin; Pochai et al. In the most abundant group, the Thoracicaadult specimens are permanently attached to various types of substrates, other living organisms e.

Although part of the global epicentre of marine biodiversity, knowledge of the barnacle fauna of the Moluccas is relatively sparse. The exploration of the natural history of the Moluccas dates back to the 17 th century, starting with Georg Everhard Rumphius, and later, for example, Alfred Russel Wallace Strack ; Lamoureux In Find horny lonely girls need company Glen Burnie 19 th and 20 th centuries, there were approximately fifty scientific expeditions passing through or specifically targeting the area, such as the British Challenger —the Dutch Siboga — and the Snellius — expeditions Lamoureux The most recent being the French Karubar expedition in Crosnier et al.

Map of the Moluccan Islands Maluku in grey in eastern Indonesia.

Map modified from Shorthouse Moluccan barnacles have not been studied since Buckeridge examined some material from the Karubar expedition. To contribute to the taxonomic knowledge of this understudied area, we herein present the first checklist of barnacles from the Moluccas, including additional information on morphology and molecular data, as well as distribution and substrate.

Specimens examined in this study were collected by the first author during 19 field trips between January and September to the intertidal zones of the Moluccan islands of Ambon, Saparua, Seram, Pombo, and Banda Neira Fig. Additional material used for the molecular analyses was collected from the island of Sulawesi in September to October New in townlooking for new friendships. In total, lots containing 1, specimens were collected from 51 Moluccan localities.

Annotated checklist tabulation of barnacle species from the Moluccas, Eastern Indonesia. Barnacle specimens firmly attached to hard substrate rocks, stone, concrete were sampled using a chisel and hammer whereas those attached to softer substrate using a craft knife.

Barnacle Want to try something fun attached to other barnacle species were not separated except for specimens dissected and measured but were kept within the same glass container, enabling further morphological studies of different species attached to each other, e. Specimens were studied by the first author.

All species were determined based on external shell morphology, Looking Brook Park for naughty single women the pattern of the parietes, opercular plates, mouth parts, and arthropodal characters, as described by Darwin, Hoek, Southward and NewmanChan et al.

Hard body parts parietes and opercular plates were separated from soft body parts using a scalpel. Shell plates were separated and cleaned with a bleach solution to remove any organic material, rinsed with fresh water, dried and observed under a stereo microscope Leica M and photographed with a digital camera Leica Microsystems MC and Leica Z16 APo-A Fig.

External morphology of barnacles showing the two parts of the operculum tergum and scutum and carina without scale. The mouthparts labrum, palps, maxilla, maxillule, and mandible were dissected using a scalpel, each was mounted on a glass slide and examined under a light microscope Axioskop The cirri were separated into couples of cirri I—VI and the penis, before being mounted on glass slides.

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The anatomy of these soft body parts was studied using a light microscope. All measurements were made using digital callipers accurate to 0.

For deep-water barnacles, capitular height, capitular width, peduncular length, orifice height, of crests, capitular thickness, and peduncular width were taken following the method described by Chan et al. We performed molecular phylogenetic analyses including new DNA sequences from American singles dating site new samples from the Moluccas in combination with sequences of multiple barnacle specimens retrieved from GenBank.

Our aim with the molecular analyses was not to provide a robust phylogeny of barnacles or to develop DNA barcodes for Moluccan barnacle taxa. Instead, our goal was to confirm the molecular taxonomic identity of the barnacles from the Phone numbers for horny women in Auburn with the published sequences in the GenBank, to examine whether they cluster near to congeneric or conspecific accessions. With this exercise we aimed to gain insights into the taxonomic positions of Moluccan barnacles in addition to those we may gain from morphology.

We sequenced two gene fragments: cytochrome oxidase subunit I COIa rapidly evolving gene from the mitochondrial genome; and the 18S ribosomal RNA gene 18Sa slowly evolving and generally highly conserved gene from the ribosome. PCR products were sent to Macrogen Europe for cycle sequencing of both strands of each gene.

Associated data

Chromatograms were edited using CodonCode Aligner version 5. All new DNA sequences generated for this study are deposited in GenBank under the accession s provided in Suppl. Sequences were aligned using Muscle Edgar as implemented in Geneious and later exported as nexus or fasta files. Bayesian phylogeny of Women looking sex tonight Lakeside gene sequences. Families with relevance for this study are highlighted by coloured rectangles.

Sample labels in red indicate sequences newly generated for this study. Species names in bold indicate potential new species. Bayesian phylogeny of COI gene sequences. As our goal was to obtain a topology and not dates for the branching events, we used a Bayesian relaxed lognormal clock with a rate Does anyone actually date anymore 1.

Original research article

Therefore, the ages obtained in the ultrametric trees emerging from this analysis are Bbw whores Hollansburg ohio not absolute. For each analysis, we ran two independent chains of between 10 and 40 million generations, with a birth-death tree prior. Convergence of chains and burn-ins were assessed with Tracer, runs combined using LogCombiner, and maximum clade credibility trees produced in Tree Annotator. The morphological analyses of the collected material revealed 24 intertidal species and one deep-water species from three families of stalked barnacles Heteralepadidae : Worcester bbw chat room genus and species; Lepadidae : two genera and species; Pollicipedidae : one genus and species and four families of acorn barnacles Pachylasmatidae : one genus and species; Chthamalidae : five genera and species; Tetraclitidae : five genera and eight species; Balanidae : two genera and seven species.

Of these, 21 are new records and two Amphibalanus sp. In general, the smallest species was C. Subclass Thecostraca Gruvel, Superorder Thoracica Darwin, Suborder Heteralepadomorpha Newman, Family Heteralepadidae Nilsson-Cantell, Genus Heteralepas Pilsbry, Figure 3a—gTable 1: species no.

Heteralepas japonica Aurivillius, MZB Cru Cir 2 a side view Older man seeking younger women for pussy Ketchikan the capitulum and peduncle b upper view showing the capitulum and peduncle c side view showing the opening of operculum d cirrus I showing the filamentary appendage at the basal region e cirrus VI showing the caudal appendage f mandible g maxillule.

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Alepas japonica Aurivillius, Aurivillius 28, pl. II figs 14, 15, pl. VIII figs 3, 7, pl. IX fig. Alepas indica Gruvel, Gruvel a:fig.

Heteralepas Heteralepas japonica : Pilsbry a: Heteralepas Heteralepas japonica var. Heteralepas Heteralepas dubia Broch,fig. Heteralepas japonica : Pilsbry a: 71, fig.